Cellosaurus logo
expasy logo

Cellosaurus MDCK-WT-EKARrEV-NLS-LDR-mRFP-FKBP-mSos1-linkercat (CVCL_D5JR)

[Text version]
Cell line name MDCK-WT-EKARrEV-NLS-LDR-mRFP-FKBP-mSos1-linkercat
Accession CVCL_D5JR
Resource Identification Initiative To cite this cell line use: MDCK-WT-EKARrEV-NLS-LDR-mRFP-FKBP-mSos1-linkercat (RRID:CVCL_D5JR)
Comments Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal as well a construct containing a Lyn-targeted (first 11 residues of LYN) rapamycin-binding (FRB) domain from MTOR (LDR) and a third construct pT2Aneo-mRFP-FKBP-mSos1-linkercat (PubMed=34667080).
Genetic integration: Method=Transduction; Gene=HGNC; 3942; MTOR (Note=With Met1_Arg2018del and Gln2114_Trp2549del = FRB).
Genetic integration: Method=Transduction; Gene=HGNC; 3711; FKBP1A.
Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_D5J1 (MDCK-WT-EKARrEV-NLS-LDR)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=34667080; DOI=10.26508/lsa.202101206; PMCID=PMC8548211
Lin S.-H., Hirayama D., Maryu G., Matsuda K., Hino N., Deguchi E., Aoki K., Iwamoto R., Terai K., Matsuda M.
Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration.
Life Sci. Alliance 5:e202101206.1-e202101206.13(2022)

Cross-references
Encyclopedic resources Wikidata; Q127382792
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2