ID   MDCK-EKAREV-NLS
AC   CVCL_D5IG
DR   Wikidata; Q127382784
RX   PubMed=27936234;
CC   Characteristics: Transduced with the FRET biosensor EKAREV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from Cdc25, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=27936234).
CC   Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
CC   Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
OX   NCBI_TaxID=9615; ! Canis lupus familiaris (Dog)
HI   CVCL_0422 ! MDCK
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
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RX   PubMed=27936234; DOI=10.1371/journal.pone.0167940; PMCID=PMC5148048;
RA   Kawabata N., Matsuda M.;
RT   "Cell density-dependent increase in tyrosine-monophosphorylated ERK2
RT   in MDCK cells expressing active Ras or Raf.";
RL   PLoS ONE 11:E0167940-E0167940(2016).
//