ID   S2R+-NPT005deltaDsh7deltaStbm8deltaFz16
AC   CVCL_D5B5
SY   S2R+-NPT005deltaDshdeltaStbmdeltaFz; S2R+-NPT005 TKO
DR   DGRC; 347
DR   FlyBase_Cell_line; FBtc0000347
DR   Wikidata; Q127384163
RX   PubMed=37995695;
CC   Group: Insect cell line.
CC   Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0000499; dsh.
CC   Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0001085; fz.
CC   Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0015838; Vang.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_D5XX ! S2R+-NPT005deltaDsh7deltaStbm8
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
//
RX   PubMed=37995695; DOI=10.1016/j.cub.2023.10.071; PMCID=PMC7616066;
RA   Strutt H., Warrington S., Madathil A.C.K., Langenhan T., Strutt D.;
RT   "Molecular symmetry breaking in the Frizzled-dependent planar polarity
RT   pathway.";
RL   Curr. Biol. 33:5340-5354.e6(2023).
//