ID   N27
AC   CVCL_D584
SY   N-27; 1RB3AN27; 1RB3A-N27
DR   BTO; BTO_0002393
DR   Millipore; SCC048
DR   Wikidata; Q54907338
RX   PubMed=7820310;
RX   PubMed=27512998;
CC   Characteristics: Used as a dopaminergic neuron model for in vitro and in vivo studies (Millipore=SCC048).
CC   Characteristics: During many passages over 20 years, the original cell line became a mixture of cell types with less than 5% TH-positive dopamine neurons (PubMed=27512998).
CC   Doubling time: 24 hours (PubMed=27512998).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
CC   Derived from site: In situ; Embryonic brain, mesencephalon; UBERON=UBERON_0009616.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
SX   Sex unspecified
AG   12FD
CA   Transformed cell line
DT   Created: 22-10-12; Last updated: 19-12-24; Version: 15
//
RX   PubMed=7820310; DOI=10.1007/BF02631258;
RA   Prasad K.N., Carvalho E., Kentroti S., Edwards-Prasad J., Freed C.R.,
RA   Vernadakis A.;
RT   "Establishment and characterization of immortalized clonal cell lines
RT   from fetal rat mesencephalic tissue.";
RL   In Vitro Cell. Dev. Biol. Anim. 30:596-603(1994).
//
RX   PubMed=27512998; DOI=10.1371/journal.pone.0160847; PMCID=PMC4981411;
RA   Gao L., Zhou W.-B., Symmes B., Freed C.R.;
RT   "Re-cloning the N27 dopamine cell line to improve a cell culture model
RT   of Parkinson's disease.";
RL   PLoS ONE 11:e0160847.1-e0160847.18(2016).
//