ID   HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2
AC   CVCL_D4BZ
SY   Syn10 split mNeonGreen
DR   Wikidata; Q127381682
CC   Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line).
CC   Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of a split mNeonGreen (3K variant, B-strands 1-10) as well as an mKate2 marker (Direct_author_submission).
CC   Genetic integration: Method=Transduction; Gene=HGNC; 11138; SNCA (Note=With p.Ala53Thr).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=FPbase; DBBO8; mKate2 (Note=Bright red fluorescent protein derived from eqFP578).
CC   Genetic integration: Method=Transduction; Gene=FPbase; ZRKRV; mNeonGreen (Note=Derivative of Branchiostoma lanceolatum LanYFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D4BW ! HEK293T TetOff-SNCA-A53T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 30-01-24; Last updated: 10-09-24; Version: 2
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