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Cellosaurus HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 (CVCL_D4BX)

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Cell line name HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3
Synonyms Syn10 Galectin-3 FRET
Accession CVCL_D4BX
Resource Identification Initiative To cite this cell line use: HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 (RRID:CVCL_D4BX)
Comments Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line).
Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11138; SNCA (Note=With p.Ala53Thr).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:6563; LGALS3.
Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transduction; Gene=FPbase; MZMFB; mRuby3 (Note=Bright red fluorescent protein derived from eqFP611).
Genetic integration: Method=Transduction; Gene=FPbase; RYFE4; mClover3 (Note=Basic green/yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D4BW (HEK293T TetOff-SNCA-A53T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Cross-references
Encyclopedic resources Wikidata; Q127381683
Entry history
Entry creation30-Jan-2024
Last entry update19-Dec-2024
Version number3