Cellosaurus HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 (CVCL_D4BX)
| Cell line name | HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 |
|---|---|
| Synonyms | Syn10 Galectin-3 FRET |
| Accession | CVCL_D4BX |
| Resource Identification Initiative | To cite this cell line use: HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 (RRID:CVCL_D4BX) |
| Comments | Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission). Genetic integration: Method=Transduction; Gene=HGNC; 11138; SNCA (Note=With p.Ala53Thr). Genetic integration: Method=Transduction; Gene=HGNC; 6563; LGALS3. Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transduction; Gene=UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby3). Genetic integration: Method=Transduction; Gene=FPbase; RYFE4; mClover3 (Note=Basic green/yellow fluorescent protein derived from GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_D4BW (HEK293T TetOff-SNCA-A53T) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q127381683 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 2 |