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Cellosaurus HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3 (CVCL_D4BV)

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Cell line name HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3
Synonyms HEK alpha-synuclein (SNCA) FRET reporter
Accession CVCL_D4BV
Resource Identification Initiative To cite this cell line use: HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3 (RRID:CVCL_D4BV)
Comments Characteristics: Transfected with two plasmids encoding mRuby3- and mClover3-tagged SNCA p.Ala53Thr expressed under the control of CMV promoters (Direct_author_submission).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11138; SNCA (Note=With p.Ala53Thr).
Genetic integration: Method=Transfection; Gene=FPbase; MZMFB; mRuby3 (Note=Bright red fluorescent protein derived from eqFP611).
Genetic integration: Method=Transfection; Gene=FPbase; RYFE4; mClover3 (Note=Basic green/yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Cross-references
Encyclopedic resources Wikidata; Q127381679
Entry history
Entry creation30-Jan-2024
Last entry update19-Dec-2024
Version number3