Cellosaurus HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3 (CVCL_D4BV)
| Cell line name | HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3 |
|---|---|
| Synonyms | HEK alpha-synuclein (SNCA) FRET reporter |
| Accession | CVCL_D4BV |
| Resource Identification Initiative | To cite this cell line use: HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3 (RRID:CVCL_D4BV) |
| Comments | Characteristics: Transfected with two plasmids encoding mRuby3- and mClover3-tagged SNCA p.Ala53Thr expressed under the control of CMV promoters (Direct_author_submission). Genetic integration: Method=Transfection; Gene=HGNC; 11138; SNCA (Note=With p.Ala53Thr). Genetic integration: Method=Transfection; Gene=UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby3). Genetic integration: Method=Transfection; Gene=FPbase; RYFE4; mClover3 (Note=Basic green/yellow fluorescent protein derived from GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0063 (HEK293T) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q127381679 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 2 |