Cellosaurus Flp-In-T-REx-293 eGFP-mHCN2 (CVCL_D4B1)
| Cell line name | Flp-In-T-REx-293 eGFP-mHCN2 |
|---|---|
| Accession | CVCL_D4B1 |
| Resource Identification Initiative | To cite this cell line use: Flp-In-T-REx-293 eGFP-mHCN2 (RRID:CVCL_D4B1) |
| Comments | Characteristics: Using pcDNA5/FRT/TO and pOG44, mouse Hcn2 N-terminally tagged with eGFP was integrated in a single site and its expression is regulated by tetracycline (PubMed=38377199). Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:1298210; Hcn2. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_U427 (Flp-In-T-REx-293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=38377199; DOI=10.1073/pnas.2315132121; PMCID=PMC10907242 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q127380859 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 3 |