ID   CH-R1.41
AC   CVCL_D3X4
SY   R1.41
DR   Wikidata; Q127380440
RX   PubMed=7559470;
CC   Characteristics: This is a class II diphtheria toxin resistant cell line. The resistance of class II mutants cannot be overcome by increased concentration of the toxin. They possess a mutationally altered EF-2 which does not serve as a substrate for ADP-ribosylation by diphtheria toxin.
CC   Selected for resistance to: UniProtKB; P00588; Corynephage beta diphtheria toxin.
CC   Sequence variation: Mutation; UniProtKB; P09445; EEF2; Simple; p.Ile714Asn (c.2141T>A); Zygosity=Heterozygous (PubMed=7559470).
CC   Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_D3VX ! CHO-K1c
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 30-01-24; Last updated: 10-09-24; Version: 2
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RX   PubMed=7559470; DOI=10.1074/jbc.270.39.23218;
RA   Foley B.T., Moehring J.M., Moehring T.J.;
RT   "Mutations in the elongation factor 2 gene which confer resistance to
RT   diphtheria toxin and Pseudomonas exotoxin A. Genetic and biochemical
RT   analyses.";
RL   J. Biol. Chem. 270:23218-23225(1995).
//