Cellosaurus logo
expasy logo

Cellosaurus CH-K1W1.P17 (CVCL_D3X3)

[Text version]
Cell line name CH-K1W1.P17
Synonyms K1W1.P17
Accession CVCL_D3X3
Resource Identification Initiative To cite this cell line use: CH-K1W1.P17 (RRID:CVCL_D3X3)
Comments Characteristics: This is a class II Pseudomonas toxin resistant cell line. The resistance of class II mutants cannot be overcome by increased concentration of the toxin. They possess a mutationally altered EF-2 which does not serve as a substrate for ADP-ribosylation by the Pseudomonas toxin.
Selected for resistance to: UniProtKB; P11439; Pseudomonas aeruginosa exotoxin A (eta).
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; P09445; EEF2; Simple; p.Gly719Asp (c.2156G>A); Zygosity=Heterozygous (PubMed=7559470).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_D3VX (CHO-K1c)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=7559470; DOI=10.1074/jbc.270.39.23218
Foley B.T., Moehring J.M., Moehring T.J.
Mutations in the elongation factor 2 gene which confer resistance to diphtheria toxin and Pseudomonas exotoxin A. Genetic and biochemical analyses.
J. Biol. Chem. 270:23218-23225(1995)

Cross-references
Encyclopedic resources Wikidata; Q127380433
Entry history
Entry creation30-Jan-2024
Last entry update19-Dec-2024
Version number3