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Cellosaurus CH-RPE.11a (CVCL_D3WW)

[Text version]
Cell line name CH-RPE.11a
Synonyms RPE.11a
Accession CVCL_D3WW
Resource Identification Initiative To cite this cell line use: CH-RPE.11a (RRID:CVCL_D3WW)
Comments Characteristics: This is a class II Pseudomonas toxin resistant cell line. The resistance of class II mutants cannot be overcome by increased concentration of the toxin. They possess a mutationally altered EF-2 which does not serve as a substrate for ADP-ribosylation by the Pseudomonas toxin (PubMed=494060).
Selected for resistance to: UniProtKB; P11439; Pseudomonas aeruginosa exotoxin A (eta).
Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; P09445; EEF2; Simple; p.Ser584Gly (c.1750A>G); Zygosity=Heterozygous (PubMed=7559470).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=494060; DOI=10.1007/BF01538880
Moehring J.M., Moehring T.J.
Characterization of the diphtheria toxin-resistance system in Chinese hamster ovary cells.
Somatic Cell Genet. 5:453-468(1979)

PubMed=7559470; DOI=10.1074/jbc.270.39.23218
Foley B.T., Moehring J.M., Moehring T.J.
Mutations in the elongation factor 2 gene which confer resistance to diphtheria toxin and Pseudomonas exotoxin A. Genetic and biochemical analyses.
J. Biol. Chem. 270:23218-23225(1995)

Cross-references
Encyclopedic resources Wikidata; Q127380458
Entry history
Entry creation30-Jan-2024
Last entry update10-Sep-2024
Version number2