ID   CH-RE1.22c
AC   CVCL_D3WG
SY   RE1.22c
DR   Wikidata; Q127380446
RX   PubMed=291128;
RX   PubMed=494060;
RX   PubMed=1353910;
RX   PubMed=6928655;
RX   PubMed=7559470;
CC   Characteristics: This is a class II diphtheria toxin resistant cell line. The resistance of class II mutants cannot be overcome by increased concentration of the toxin. They possess a mutationally altered EF-2 which does not serve as a substrate for ADP-ribosylation by diphtheria toxin (PubMed=494060).
CC   Selected for resistance to: UniProtKB; P00588; Corynephage beta diphtheria toxin.
CC   Sequence variation: Mutation; UniProtKB; P09445; EEF2; Simple; p.Gly717Arg (c.2149G>A); Zygosity=Homozygous (PubMed=1353910).
CC   Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_D3X1 ! CH-RE1.22
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 30-01-24; Last updated: 10-09-24; Version: 2
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RX   PubMed=291128; DOI=10.1007/BF01538881;
RA   Moehring T.J., Danley D.E., Moehring J.M.;
RT   "Codominant translational mutants of Chinese hamster ovary cells
RT   selected with diphtheria toxin.";
RL   Somatic Cell Genet. 5:469-480(1979).
//
RX   PubMed=494060; DOI=10.1007/BF01538880;
RA   Moehring J.M., Moehring T.J.;
RT   "Characterization of the diphtheria toxin-resistance system in Chinese
RT   hamster ovary cells.";
RL   Somatic Cell Genet. 5:453-468(1979).
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RX   PubMed=1353910; DOI=10.1007/BF01233859;
RA   Foley B.T., Moehring J.M., Moehring T.J.;
RT   "A mutation in codon 717 of the CHO-K1 elongation factor 2 gene
RT   prevents the first step in the biosynthesis of diphthamide.";
RL   Somat. Cell Mol. Genet. 18:227-231(1992).
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RX   PubMed=6928655; DOI=10.1073/pnas.77.2.1010; PMCID=PMC348413;
RA   Moehring J.M., Moehring T.J., Danley D.E.;
RT   "Posttranslational modification of elongation factor 2 in
RT   diphtheria-toxin-resistant mutants of CHO-K1 cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:1010-1014(1980).
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RX   PubMed=7559470; DOI=10.1074/jbc.270.39.23218;
RA   Foley B.T., Moehring J.M., Moehring T.J.;
RT   "Mutations in the elongation factor 2 gene which confer resistance to
RT   diphtheria toxin and Pseudomonas exotoxin A. Genetic and biochemical
RT   analyses.";
RL   J. Biol. Chem. 270:23218-23225(1995).
//