Cellosaurus BU2:N1 (CVCL_D2WH)
| Cell line name | BU2:N1 |
|---|---|
| Synonyms | BU2 N1; BU2-TetOn:NICD |
| Accession | CVCL_D2WH |
| Resource Identification Initiative | To cite this cell line use: BU2:N1 (RRID:CVCL_D2WH) |
| Comments | From: Boston University School of Medicine; Boston; USA. Population: Caucasian. Characteristics: Under the control of doxycycline expresses the intracellular domain of the NOTCH1 receptor (NICD1). Using ZFN a construct containing a reverse tetracycline-controlled transactivator (rtTA), NICD1 and puroR was inserted in the AAVS1 safe harbor locus (PubMed=36332629). Genetic integration: Method=ZFN; Gene=HGNC; 7881; NOTCH1 (Note=With p.Met1_Arg1762del = NICD1). Genetic integration: Method=ZFN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=ZFN; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=ZFN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_JW25 (CREM002i-BU2C10) |
| Sex of cell | Male |
| Age at sampling | 45Y |
| Category | Induced pluripotent stem cell |
| Web pages | https://stemcellbank.bu.edu/Catalog/Item/Details/782 |
| Publications | PubMed=36332629; DOI=10.1016/j.stemcr.2022.10.007; PMCID=PMC9768581 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q127380349 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 2 |