ID   A549-AC
AC   CVCL_D1JI
RX   PubMed=36032971;
CC   Group: SARS-CoV-2 research cell line.
CC   Population: Caucasian.
CC   Characteristics: Expresses ACE2 under the control of the EF1a promoter (from parent cell line).
CC   Characteristics: Tranduced with a EF1a-Cas9-2A-eGFP vector (PubMed=36032971).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
CC   Transfected with: HGNC; 13557; ACE2.
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Omics: CRISPR phenotypic screen.
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
DI   NCIt; C3512; Lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A7UV ! A549-hACE2 [BEI resources]
SX   Male
AG   58Y
CA   Cancer cell line
DT   Created: 30-01-24; Last updated: 30-01-24; Version: 1
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RX   PubMed=36032971; DOI=10.21203/rs.3.rs-1910932/v1;
RA   Hou J.-K., Wei Y.-J., Zou J., Jaffery R., Liang S.-H., Zheng C.-S.,
RA   Chen K., Shi P.-Y., Chen Y.-W., Xie X.-P., Peng W.-Y.;
RT   "Integrated multi-omics analyses identify key anti-viral host factors
RT   and pathways controlling SARS-CoV-2 infection.";
RL   Res. Sq. 2022:1910932-1910932(2022).
//