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Cellosaurus HEK293::TMEM192-3xHA RB1CC1-/- mNEON-YIPF4 (CVCL_D1JA)

[Text version]
Cell line name HEK293::TMEM192-3xHA RB1CC1-/- mNEON-YIPF4
Synonyms HEK293::TMEM192-3xHA FIP200-/- ::mNEON-YIPF4; HEK293 FIP200-/-
Accession CVCL_D1JA
Resource Identification Initiative To cite this cell line use: HEK293::TMEM192-3xHA RB1CC1-/- mNEON-YIPF4 (RRID:CVCL_D1JA)
Comments Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line).
Characteristics: Using CRISPR/Cas9 YIPF4 was N-terminally tagged on both alleles with mNeonGreen (PubMed=37757899).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:15574; RB1CC1.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZRKRV; mNeonGreen (Note=Derivative of Branchiostoma lanceolatum LanYFP).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D1KQ (HEK293::TMEM192-3xHA RB1CC1-/-)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=37757899; DOI=10.1038/s41586-023-06657-6; PMCID=PMC10620096
Hickey K.L., Swarup S., Smith I.R., Paoli J.C., Whelan E.M., Paulo J.A., Harper J.W.
Proteome census upon nutrient stress reveals Golgiphagy membrane receptors.
Nature 623:167-174(2023)

Cross-references
Encyclopedic resources Wikidata; Q127381649
Entry history
Entry creation30-Jan-2024
Last entry update19-Dec-2024
Version number3