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Cellosaurus H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/- (CVCL_D1ID)

[Text version]
Cell line name H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/-
Synonyms H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; H9 FAM134A/B/C/TEX264-/-
Accession CVCL_D1ID
Resource Identification Initiative To cite this cell line use: H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/- (RRID:CVCL_D1ID)
Comments Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:25964; RETREG1.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:28450; RETREG2.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:27258; RETREG3.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:30247; TEX264.
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D1IC (H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/-)
Children:
CVCL_D1IV (H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/- Keima-RAMP4)CVCL_D1IW (H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/- Keima-REEP5)CVCL_D1IE (H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/- CCPG1-/-)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=38429475; DOI=10.1038/s41556-024-01356-4; PMCID=PMC10940164
Hoyer M.J., Capitanio C., Smith I.R., Paoli J.C., Bieber A., Jiang Y.-Z., Paulo J.A., Gonzalez-Lozano M.A., Baumeister W.P., Wilfling F., Schulman B.A., Harper J.W.
Combinatorial selective ER-phagy remodels the ER during neurogenesis.
Nat. Cell Biol. 26:378-392(2024)

Cross-references
Encyclopedic resources Wikidata; Q127381003
Entry history
Entry creation30-Jan-2024
Last entry update19-Dec-2024
Version number3