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Cellosaurus S2 PfRH5 variant 2 (CVCL_D1FU)

[Text version]
Cell line name S2 PfRH5 variant 2
Synonyms S2 PfRH5 v2.0
Accession CVCL_D1FU
Resource Identification Initiative To cite this cell line use: S2 PfRH5 variant 2 (RRID:CVCL_D1FU)
Comments Group: Insect cell line.
Group: Malaria research cell line.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q8IFM5; Plasmodium falciparum (isolate 3D7) reticulocyte-binding protein homolog 5 (RH5) (PfRH5) (Note=With p.Met1_Phe25del, p.Thr40Ala, p.Thr216Ala, p.Thr286Ala, p.Thr299Ala and a C-terminal His6 tag).
Genetic integration: Method=Transfection/transduction; Gene=FlyBase_Gene; FBgn0001218; Hsc70-3 (Note=AA 1-18 = Signal sequence).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
Hierarchy Parent: CVCL_A8PY (HighMan-S2)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Publications

PubMed=27457156; DOI=10.1038/srep30357; PMCID=PMC4960544
Hjerrild K.A., Jin J., Wright K.E., Brown R.E., Marshall J.M., Labbe G.M., Silk S.E., Cherry C.J., Clemmensen S.B., Jorgensen T., Illingworth J.J., Alanine D.G.W., Milne K.H., Ashfield R., de Jongh W.A., Douglas A.D., Higgins M.K., Draper S.J.
Production of full-length soluble Plasmodium falciparum RH5 protein vaccine using a Drosophila melanogaster Schneider 2 stable cell line system.
Sci. Rep. 6:30357-30357(2016)

Cross-references
Encyclopedic resources Wikidata; Q127384145
Entry history
Entry creation30-Jan-2024
Last entry update10-Sep-2024
Version number2