ID   hIPSC-Di21-C3-5-11
AC   CVCL_D1FG
SY   Di C3-5-11; C3-5-11
DR   Wikidata; Q127381763
RX   PubMed=23084023;
CC   Population: Caucasian.
CC   Characteristics: This cell line has lot one copy of chromosome 21. Using CRISPR/Cas9 a IRES-TK-Neo-polyA site cassette was introduced in one allele of exon 3 of APP on chromosome 21 of the parent cell line. The parent cell line was cultured in the absence of G418 selection for three passages to allow chromosome loss to take place, and then were cultured in the presence of ganciclovir (GCV) to select a clone without the presence of the TK-neo construct (PubMed=23084023).
CC   Derived from site: In situ; Chest, skin; UBERON=UBERON_0001868.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
DI   NCIt; C2993; Down syndrome
DI   ORDO; Orphanet_870; Down syndrome
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D1FB ! hIPSC-Tri21-C3-5
SX   Female
AG   1Y
CA   Induced pluripotent stem cell
DT   Created: 30-01-24; Last updated: 10-09-24; Version: 2
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RX   PubMed=23084023; DOI=10.1016/j.stem.2012.08.004; PMCID=PMC3705773;
RA   Li L.B., Chang K.-H., Wang P.-R., Hirata R.K., Papayannopoulou T.,
RA   Russell D.W.;
RT   "Trisomy correction in Down syndrome induced pluripotent stem cells.";
RL   Cell Stem Cell 11:615-619(2012).
//