ID   WAe001-A-1X
AC   CVCL_C9LD
SY   H1 MYH11-NLuc-tdTomato
DR   hPSCreg; WAe001-A-1X
DR   Wikidata; Q123033809
RX   PubMed=31080110;
CC   Characteristics: Using CRISPR/Cas9 a Nanoluc-T2A-tdTomato-neo construct was integrated at the N-terminus of MYH11 (PubMed=31080110).
CC   Transfected with: NanoLuc, a 19 kDa ATP-independent bright luminescent protein derived from Oplophorus gracilirostris.
CC   Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9771 ! WA01
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 05-10-23; Last updated: 30-01-24; Version: 2
//
RX   PubMed=31080110; DOI=10.1016/j.stemcr.2019.04.013;
RA   Zhang J., McIntosh B.E., Wang B., Brown M.E., Probasco M.D.,
RA   Webster S., Duffin B., Zhou Y., Guo L.-W., Burlingham W.J., Kent C.,
RA   Ferris M., Thomson J.A.;
RT   "A human pluripotent stem cell-based screen for smooth muscle cell
RT   differentiation and maturation identifies inhibitors of intimal
RT   hyperplasia.";
RL   Stem Cell Reports 12:1269-1281(2019).
//