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Cellosaurus DU145/Cas9-hyg (CVCL_C9GA)

[Text version]
Cell line name DU145/Cas9-hyg
Accession CVCL_C9GA
Resource Identification Initiative To cite this cell line use: DU145/Cas9-hyg (RRID:CVCL_C9GA)
Comments Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL548).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: Metastatic; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Asp84Tyr (c.250G>T) (p.Arg98Leu, c.293G>T); ClinVar=VCV000376306; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9884; RB1; Simple; p.Lys715Ter (c.2143A>T); ClinVar=VCV001786615; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Lys178fs*86 (c.532_536delAAGCC); Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Pro223Leu (c.668C>T); ClinVar=VCV000234214; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Val274Phe (c.820G>T); ClinVar=VCV000376674; Zygosity=Heterozygous (from parent cell line).
Disease Prostate carcinoma (NCIt: C4863)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0105 (DU145)
Sex of cell Male
Age at sampling 69Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL548
Encyclopedic resources Wikidata; Q123031192
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number4