Cellosaurus cell line SNU-475/Cas9-hyg (CVCL

Cellosaurus SNU-475/Cas9-hyg (CVCL_C9G6)

Cross-references
Cell line name	SNU-475/Cas9-hyg
Accession	CVCL_C9G6
Resource Identification Initiative	To cite this cell line use: SNU-475/Cas9-hyg (RRID:CVCL_C9G6)
Comments	Population: Korean. Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL522). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations	Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line). Mutation; HGNC; 11998; TP53; Simple; p.Asn239Asp (c.715A>G); ClinVar=VCV000234036; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 11998; TP53; Simple; p.Gly262Asp (c.785G>A); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 11998; TP53; Simple; p.Cys275Arg (c.823T>C); ClinVar=VCV000376584; Zygosity=Unspecified (from parent cell line). Mutation; HGNC; 11998; TP53; Simple; p.Asn288Ser (c.863A>G); Zygosity=Unspecified (from parent cell line).
Disease	Adult hepatocellular carcinoma (NCIt: C7956) Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0497 (SNU-475)
Sex of cell	Male
Age at sampling	43Y
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL522
Encyclopedic resources	Wikidata; Q123033544
Entry history
Entry creation	05-Oct-2023
Last entry update	30-Jan-2024
Version number	2