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Cellosaurus NIH/3T3/Cas9-hyg (CVCL_C9FL)

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Cell line name NIH/3T3/Cas9-hyg
Synonyms NIH/3T3-Cas9-hyg
Accession CVCL_C9FL
Resource Identification Initiative To cite this cell line use: NIH/3T3/Cas9-hyg (RRID:CVCL_C9FL)
Comments Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL569).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Parent: CVCL_0594 (NIH 3T3)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL569
Encyclopedic resources Wikidata; Q123033187
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number4