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Cellosaurus CT26/Cas9-hyg (CVCL_C9FK)

[Text version]
Cell line name CT26/Cas9-hyg
Synonyms CT26-Cas9-hyg
Accession CVCL_C9FK
Resource Identification Initiative To cite this cell line use: CT26/Cas9-hyg (RRID:CVCL_C9FK)
Comments Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL582).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: ChEBI; CHEBI:82373; N-nitroso-N-methylurethane (NNMU).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Gene deletion; MGI; MGI:104738; Cdkn2a; Zygosity=Homozygous (from parent cell line).
  • Mutation; MGI; MGI:96680; Kras; Simple; p.Gly12Asp (c.35G>A); Zygosity=Homozygous (from parent cell line).
Disease Mouse colon adenocarcinoma (NCIt: C120044)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_7254 (CT26)
Sex of cell Female
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL582
Encyclopedic resources Wikidata; Q123030979
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3