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Cellosaurus 4T1/Cas9-hyg (CVCL_C9FJ)

[Text version]
Cell line name 4T1/Cas9-hyg
Synonyms 4T1-Cas9-hyg
Accession CVCL_C9FJ
Resource Identification Initiative To cite this cell line use: 4T1/Cas9-hyg (RRID:CVCL_C9FJ)
Comments Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL581).
Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Disease Malignant neoplasms of the mouse mammary gland (NCIt: C21678)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cfC3H.
Hierarchy Parent: CVCL_0125 (4T1)
Sex of cell Female
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL581
Encyclopedic resources Wikidata; Q123030297
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3