Cellosaurus HEK293/HF-Cas9-AAVS1 (CVCL_C9FA)
| Cell line name | HEK293/HF-Cas9-AAVS1 |
|---|---|
| Accession | CVCL_C9FA |
| Resource Identification Initiative | To cite this cell line use: HEK293/HF-Cas9-AAVS1 (RRID:CVCL_C9FA) |
| Comments | Characteristics: Stably expresses a CMV-3xFLAG-HFCas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL553). Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asn497Ala, p.Arg661Ala, p.Gln695Ala and p.Gln926Ala = High-fidelity Cas9). Genetic integration: Method=Transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata). Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Cross-references | |
| Cell line collections (Providers) | GeneCopoeia; SL553 |
| Encyclopedic resources | Wikidata; Q123031587 |
| Entry history | |
| Entry creation | 05-Oct-2023 |
| Last entry update | 19-Dec-2024 |
| Version number | 4 |