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Cellosaurus U87-MG/Cas9 AAVS1 (CVCL_C9F7)

[Text version]
Cell line name U87-MG/Cas9 AAVS1
Synonyms U87-MG/Cas9-AAVS1
Accession CVCL_C9F7
Resource Identification Initiative To cite this cell line use: U87-MG/Cas9 AAVS1 (RRID:CVCL_C9F7)
Comments Problematic cell line: Misidentified. Parent cell line (U-87MG ATCC) is not the original glioblastoma cell line established in 1968 at the University of Uppsala.
Population: Caucasian.
Characteristics: Stably expresses a CBh-3xFLAG-Cas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL584).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Sequence variations
  • Mutation; HGNC; 7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0022 (U-87MG ATCC)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL584
Encyclopedic resources Wikidata; Q123033672
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3