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Cellosaurus 1321N1/Cas9-hyg (CVCL_C9F6)

[Text version]
Cell line name 1321N1/Cas9-hyg
Accession CVCL_C9F6
Resource Identification Initiative To cite this cell line use: 1321N1/Cas9-hyg (RRID:CVCL_C9F6)
Comments Problematic cell line: Contaminated. Ancestral cell line (U-118MG) has been shown to be a U-138MG derivative.
Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL576).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Mutation; HGNC; 9588; PTEN; Simple; c.1026+1G>T (IVS8+1G>T); ClinVar=VCV000635377; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg213Gln (c.638G>A); ClinVar=VCV000135359; Zygosity=Homozygous (from parent cell line).
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0110 (1321N1)
Sex of cell Male
Age at sampling 47Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL576
Encyclopedic resources Wikidata; Q123030220
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3