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Cellosaurus LS411N/Cas9-hyg (CVCL_C9EX)

[Text version]
Cell line name LS411N/Cas9-hyg
Accession CVCL_C9EX
Resource Identification Initiative To cite this cell line use: LS411N/Cas9-hyg (RRID:CVCL_C9EX)
Comments Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL532).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Cecum; UBERON=UBERON_0001153.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Gln789Ter (c.2365C>T); ClinVar=VCV000092341; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Cys105Valfs*8 (c.313delT); ClinVar=VCV000940879; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Lys164Argfs*3 (c.491delA); ClinVar=VCV000189448; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
Disease Cecum adenocarcinoma (NCIt: C5543)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1385 (LS411N)
Sex of cell Male
Age at sampling 32Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL532
Encyclopedic resources Wikidata; Q123032977
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3