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Cellosaurus T84/Cas9-hyg (CVCL_C9EU)

[Text version]
Cell line name T84/Cas9-hyg
Accession CVCL_C9EU
Resource Identification Initiative To cite this cell line use: T84/Cas9-hyg (RRID:CVCL_C9EU)
Comments Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL541).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: Metastatic; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Leu1488Phefs*19 (c.4464delA); Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu542Lys (c.1624G>A); ClinVar=VCV000031944; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6770; SMAD4; Simple; p.Lys340Asn (c.1020G>C); Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; c.560-1G>T; Zygosity=Homozygous; Note=Splice acceptor mutation (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0555 (T84)
Sex of cell Male
Age at sampling 72Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL541
Encyclopedic resources Wikidata; Q123033602
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3