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Cellosaurus HEK293T-CRISPRa (CVCL_C9DX)

[Text version]
Cell line name HEK293T-CRISPRa
Accession CVCL_C9DX
Resource Identification Initiative To cite this cell line use: HEK293T-CRISPRa (RRID:CVCL_C9DX)
Comments Characteristics: Transduced with lentiviral vectors dCAS-VP64_Blast (EF1a-dCas9-VP64-Bsd-WPE) and MS2-P65-HSF1_Hygro (EF1a-MS2(N55K)-SV40(NLS)-RELA-HSF1-hygR-WPRE) (PubMed=34431042).
Genetic integration: Method=Transduction; Gene=HGNC; 5224; HSF1.
Genetic integration: Method=Transduction; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368 del).
Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Omics: CRISPR phenotypic screen.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=34431042; DOI=10.1007/s11427-021-1990-5; PMCID=PMC8384091
Zhu S.-Y., Liu Y., Zhou Z., Zhang Z.-Y., Xiao X., Liu Z.-H., Chen A., Dong X.-J., Tian F., Chen S.-H., Xu Y.-Y., Wang C.-H., Li Q.-H., Niu X.-R., Pan Q., Du S., Xiao J.-Y., Wang J.-W., Wei W.-S.
Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry.
Sci. China Life Sci. 65:701-717(2022)

Cross-references
CRISP screens repositories BioGRID_ORCS_Cell_line; 1656
Encyclopedic resources Wikidata; Q123031592
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3