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Cellosaurus HPAF-II/Cas9-hyg (CVCL_C9DQ)

[Text version]
Cell line name HPAF-II/Cas9-hyg
Accession CVCL_C9DQ
Resource Identification Initiative To cite this cell line use: HPAF-II/Cas9-hyg (RRID:CVCL_C9DQ)
Comments Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL528).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
Sequence variations
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg29_Ala34del (c.85_102del18); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Pro151Ser (c.451C>T); ClinVar=VCV000012370; Zygosity=Homozygous (from parent cell line).
Disease Pancreatic ductal adenocarcinoma (NCIt: C9120)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B284 (HPAF)
Sex of cell Male
Age at sampling 44Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL528
Encyclopedic resources Wikidata; Q123031683
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number4