Cellosaurus cell line NCI-N87/Cas9-hyg (CVCL

Cross-references
Cell line name	NCI-N87/Cas9-hyg
Accession	CVCL_C9DN
Resource Identification Initiative	To cite this cell line use: NCI-N87/Cas9-hyg (RRID:CVCL_C9DN)
Comments	Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL565). Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: Metastatic; Liver; UBERON=UBERON_0002107.
Sequence variations	Gene deletion; HGNC; HGNC:6770; SMAD4; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:3431; ERBB3; Simple; p.Thr757Lys (c.2270C>A); Zygosity=Unspecified (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Homozygous (from parent cell line).
Disease	Gastric tubular adenocarcinoma (NCIt: C5473)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_1603 (NCI-N87)
Sex of cell	Male
Age at sampling	Age unspecified
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL565
Encyclopedic resources	Wikidata; Q123033160
Entry history
Entry creation	05-Oct-2023
Last entry update	19-Dec-2024
Version number	4