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Cellosaurus AGS/Cas9-hyg (CVCL_C9DJ)

[Text version]
Cell line name AGS/Cas9-hyg
Accession CVCL_C9DJ
Resource Identification Initiative To cite this cell line use: AGS/Cas9-hyg (RRID:CVCL_C9DJ)
Comments Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL520).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Stomach; UBERON=UBERON_0000945.
Sequence variations
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Gly34Glu (c.101G>A); ClinVar=VCV000017584; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu453Lys (c.1357G>A); ClinVar=VCV000376470; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Ala (c.1634A>C); ClinVar=VCV000013659; Zygosity=Heterozygous (from parent cell line).
Disease Gastric adenocarcinoma (NCIt: C4004)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0139 (AGS)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL520
Encyclopedic resources Wikidata; Q123030667
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3