Cellosaurus cell line AGS/Cas9-hyg (CVCL

Cross-references
Cell line name	AGS/Cas9-hyg
Accession	CVCL_C9DJ
Resource Identification Initiative	To cite this cell line use: AGS/Cas9-hyg (RRID:CVCL_C9DJ)
Comments	Population: Caucasian. Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL520). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: In situ; Stomach; UBERON=UBERON_0000945.
Sequence variations	Mutation; HGNC; 2514; CTNNB1; Simple; p.Gly34Glu (c.101G>A); ClinVar=VCV000017584; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu453Lys (c.1357G>A); ClinVar=VCV000376470; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Ala (c.1634A>C); ClinVar=VCV000013659; Zygosity=Heterozygous (from parent cell line).
Disease	Gastric adenocarcinoma (NCIt: C4004)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0139 (AGS)
Sex of cell	Female
Age at sampling	54Y
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL520
Encyclopedic resources	Wikidata; Q123030667
Entry history
Entry creation	05-Oct-2023
Last entry update	30-Jan-2024
Version number	2