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Cellosaurus SK-HEP-1/Cas9-hyg (CVCL_C9DI)

[Text version]
Cell line name SK-HEP-1/Cas9-hyg
Accession CVCL_C9DI
Resource Identification Initiative To cite this cell line use: SK-HEP-1/Cas9-hyg (RRID:CVCL_C9DI)
Comments Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL557).
Karyotypic information: Has lost chromosome Y (from parent cell line).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
Cell type: Endothelial cell; CL=CL_0000115.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
Disease Liver and intrahepatic bile duct epithelial neoplasm (NCIt: C7106)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0525 (SK-HEP-1)
Sex of cell Male
Age at sampling 52Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL557
Encyclopedic resources Wikidata; Q123033505
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number4