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Cellosaurus C3A/Cas9-hyg (CVCL_C9DH)

[Text version]
Cell line name C3A/Cas9-hyg
Accession CVCL_C9DH
Resource Identification Initiative To cite this cell line use: C3A/Cas9-hyg (RRID:CVCL_C9DH)
Comments Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL543).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1098 (Hep-G2/C3A)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL543
Encyclopedic resources Wikidata; Q123030840
Entry history
Entry creation05-Oct-2023
Last entry update30-Jan-2024
Version number2