Cellosaurus cell line PLC/PRF/5/Cas9-hyg (CVCL

Cellosaurus PLC/PRF/5/Cas9-hyg (CVCL_C9DF)

Cross-references
Cell line name	PLC/PRF/5/Cas9-hyg
Accession	CVCL_C9DF
Resource Identification Initiative	To cite this cell line use: PLC/PRF/5/Cas9-hyg (RRID:CVCL_C9DF)
Comments	Population: African; Shangaan. Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL531). Karyotypic information: Has lost chromosome Y (from parent cell line). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transformant: NCBI_TaxID; 10407; Hepatitis B virus (HBV). Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations	Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg112Gly (c.334C>G) (p.Pro126Arg, c.377C>G); ClinVar=VCV000233484; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; 11998; TP53; Simple; p.Arg249Ser (c.747G>T); ClinVar=VCV000012352; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; 12363; TSC2; Simple; p.Asn1651Ser (c.4952A>G); ClinVar=VCV000049335; Zygosity=Homozygous (from parent cell line).
Disease	Adult hepatocellular carcinoma (NCIt: C7956) Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0485 (PLC/PRF/5)
Sex of cell	Male
Age at sampling	24Y
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL531
Encyclopedic resources	Wikidata; Q123033273
Entry history
Entry creation	05-Oct-2023
Last entry update	30-Jan-2024
Version number	2