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Cellosaurus PLC/PRF/5/Cas9-hyg (CVCL_C9DF)

[Text version]
Cell line name PLC/PRF/5/Cas9-hyg
Accession CVCL_C9DF
Resource Identification Initiative To cite this cell line use: PLC/PRF/5/Cas9-hyg (RRID:CVCL_C9DF)
Comments Population: African; Shangaan.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL531).
Karyotypic information: Has lost chromosome Y (from parent cell line).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: NCBI_TaxID; 10407; Hepatitis B virus (HBV).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg112Gly (c.334C>G) (p.Pro126Arg, c.377C>G); ClinVar=VCV000233484; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg249Ser (c.747G>T); ClinVar=VCV000012352; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 12363; TSC2; Simple; p.Asn1651Ser (c.4952A>G); ClinVar=VCV000049335; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0485 (PLC/PRF/5)
Sex of cell Male
Age at sampling 24Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL531
Encyclopedic resources Wikidata; Q123033273
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3