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Cellosaurus BT-474-Luc2 (CVCL_C9CU)

[Text version]
Cell line name BT-474-Luc2
Accession CVCL_C9CU
Resource Identification Initiative To cite this cell line use: BT-474-Luc2 (RRID:CVCL_C9CU)
Comments Population: Caucasian.
Characteristics: Stably expresses firefly luciferase under the control of the human EF1a promoter (ATCC=HTB-20-LUC2).
Doubling time: ~61 hours (ATCC=HTB-20-LUC2).
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase (Note=With optimized codon usage for mammalian expression).
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Derived from site: In situ; Breast; UBERON=UBERON_0000310.
Sequence variations
  • Gene fusion; HGNC; HGNC:24618; AHCTF1 + HGNC; HGNC:736; NAAA; Name(s)=AHCTF1-NAAA (from parent cell line).
  • Gene fusion; HGNC; HGNC:7609; MYO9B + HGNC; HGNC:9764; RAB22A; Name(s)=RAB22A-MYO9B (from parent cell line).
  • Gene fusion; HGNC; HGNC:16173; DOK5 + HGNC; HGNC:17579; STARD3; Name(s)=STARD3-DOK5 (from parent cell line).
  • Gene fusion; HGNC; HGNC:16651; MRPL45 + HGNC; HGNC:16181; TRPC4AP; Name(s)=TRPC4AP-MRPL45 (from parent cell line).
  • Gene fusion; HGNC; HGNC:13178; IKZF3 + HGNC; HGNC:12649; VAPB; Name(s)=VAPB-IKZF3 (from parent cell line).
  • Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ser3094Ter (c.9281C>A); ClinVar=VCV000188440; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11621; HNF1A; Simple; p.Gln495Ter (c.1483C>T); ClinVar=VCV000586785; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6871; MAPK1; Simple; p.His61Gln (c.183C>G); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Lys111Asn (c.333G>C); ClinVar=VCV000376483; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Glu285Lys (c.853G>A); ClinVar=VCV000420133; Zygosity=Homozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0179 (BT-474)
Sex of cell Female
Age at sampling 60Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-20-LUC2

Markers:
AmelogeninX
CSF1PO10,11
D5S81811,13
D7S8209,12
D13S31711
D16S5399,11
TH017
TPOX8
vWA15,16

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Cross-references
Cell line collections (Providers) ATCC; HTB-20-LUC2
Encyclopedic resources Wikidata; Q123030827
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number5