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Cellosaurus MCF7/Cas9 AAVS1 (CVCL_C9CD)

[Text version]
Cell line name MCF7/Cas9 AAVS1
Accession CVCL_C9CD
Resource Identification Initiative To cite this cell line use: MCF7/Cas9 AAVS1 (RRID:CVCL_C9CD)
Comments Population: Caucasian.
Characteristics: Stably expresses a CBh-3xFLAG-Cas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL514).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0031 (MCF-7)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL514
Encyclopedic resources Wikidata; Q123033008
Entry history
Entry creation05-Oct-2023
Last entry update10-Sep-2024
Version number3