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Cellosaurus NCI-H1299/Cas9-hyg (CVCL_C9BJ)

[Text version]
Cell line name NCI-H1299/Cas9-hyg
Accession CVCL_C9BJ
Resource Identification Initiative To cite this cell line use: NCI-H1299/Cas9-hyg (RRID:CVCL_C9BJ)
Comments Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL533).
Karyotypic information: Has lost chromosome Y (from parent cell line).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000027.
Sequence variations
  • Gene deletion; HGNC; 11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Lung large cell carcinoma (NCIt: C4450)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0060 (NCI-H1299)
Sex of cell Male
Age at sampling 43Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL533
Encyclopedic resources Wikidata; Q123033153
Entry history
Entry creation05-Oct-2023
Last entry update30-Jan-2024
Version number2