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Cellosaurus HEK293T/Cas9 AAVS1 (CVCL_C9BG)

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Cell line name HEK293T/Cas9 AAVS1
Accession CVCL_C9BG
Resource Identification Initiative To cite this cell line use: HEK293T/Cas9 AAVS1 (RRID:CVCL_C9BG)
Comments Characteristics: Stably expresses a CBh-3xFLAG-Cas9-NLS-bGHPolyA-EF1a-copGFP-T2A-PuroR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL502).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL502
Encyclopedic resources Wikidata; Q123031595
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number4