ID   HepG2-NTCP1 HBV Cas9
AC   CVCL_C9BB
SY   Cas9 HepG2-HBV
DR   BioGRID_ORCS_Cell_line; 1173
DR   Wikidata; Q123031640
RX   PubMed=31801065;
CC   Population: Caucasian.
CC   Virology: Contains a stably integrated 1.3 mer copy of the Hepatitis B virus (HBV) subtype AYW genome (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; 10905; SLC10A1.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C3728; Hepatoblastoma
DI   ORDO; Orphanet_449; Hepatoblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C9BA ! HepG2-NTCP1 HBV
SX   Male
AG   15Y
CA   Cancer cell line
DT   Created: 05-10-23; Last updated: 10-09-24; Version: 3
//
RX   PubMed=31801065; DOI=10.1016/j.celrep.2019.10.113;
RA   Hyrina A., Jones C., Chen D., Clarkson S., Cochran N., Feucht P.,
RA   Hoffman G., Lindeman A., Russ C., Sigoillot F., Tsang T., Uehara K.,
RA   Xie L.-L., Ganem D.E., Holdorf M.;
RT   "A genome-wide CRISPR screen identifies ZCCHC14 as a host factor
RT   required for hepatitis B surface antigen production.";
RL   Cell Rep. 29:2970-2978.e6(2019).
//