ID   A549-deltaSTAT1-SunTag ACE2
AC   CVCL_C9AV
DR   Wikidata; Q123030362
RX   PubMed=35421191;
CC   Group: SARS-CoV-2 research cell line.
CC   Population: Caucasian.
CC   Characteristics: Doxycycline induces the expression of pCW-TRE-scFv-SunTag. SunTag consists of 24 copies of a short peptide epitope from yeast GCN4 and is used to recruite multiple protein copies to a polypeptide scaffold.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; 11362; STAT1.
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; 13557; ACE2.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Omics: CRISPR phenotypic screen.
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
DI   NCIt; C3512; Lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C9AU ! A549-deltaSTAT1-SunTag
SX   Male
AG   58Y
CA   Cancer cell line
DT   Created: 05-10-23; Last updated: 10-09-24; Version: 3
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RX   PubMed=35421191; DOI=10.1371/journal.ppat.1010464; PMCID=PMC9041830;
RA   Danziger O., Patel R.S., DeGrace E.J., Rosen M.R., Rosenberg B.R.;
RT   "Inducible CRISPR activation screen for interferon-stimulated genes
RT   identifies OAS1 as a SARS-CoV-2 restriction factor.";
RL   PLoS Pathog. 18:E1010464-E1010464(2022).
//