ID   MLL/AF9 Cas9
AC   CVCL_C8ZF
SY   Line#187
DR   BioGRID_ORCS_Cell_line; 814
DR   Wikidata; Q123033059
RX   PubMed=29478914;
CC   Characteristics: The MLL/AF9 oncogene was transduced into bone marrow hematopoietic stem cells and then transferred cells to sub-lethally irradiated recipients. Primary acute myeloid leukemia cells were harvested 3-6 months later, serially transplanted three times and then cultured in the presence of cytokines (PubMed=29478914).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Omics: CRISPR phenotypic screen.
DI   NCIt; C21604; Mouse leukemia
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Male
CA   Cancer cell line
DT   Created: 05-10-23; Last updated: 19-12-24; Version: 4
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RX   PubMed=29478914; DOI=10.1016/j.ccell.2018.01.012; PMCID=PMC5849534;
RA   Yamauchi T., Masuda T., Canver M.C., Seiler M., Semba Y., Shboul M.,
RA   Al-Raqad M., Maeda M., Schoonenberg V.A.C., Cole M.A.,
RA   Macias-Trevino C., Ishikawa Y., Yao Q.-M., Nakano M., Arai F.,
RA   Orkin S.H., Reversade B., Buonamici S., Pinello L., Akashi K.,
RA   Bauer D.E., Maeda T.;
RT   "Genome-wide CRISPR-Cas9 screen identifies leukemia-specific
RT   dependence on a pre-mRNA metabolic pathway regulated by DCPS.";
RL   Cancer Cell 33:386-400.e5(2018).
//