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Cellosaurus CALM/AF10 Cas9 (CVCL_C8ZE)

[Text version]
Cell line name CALM/AF10 Cas9
Synonyms Line #171
Accession CVCL_C8ZE
Resource Identification Initiative To cite this cell line use: CALM/AF10 Cas9 (RRID:CVCL_C8ZE)
Comments Characteristics: The CALM/AF10 oncogene was transduced into bone marrow hematopoietic stem cells and then transferred cells to sub-lethally irradiated recipients. Primary acute myeloid leukemia cells were harvested 3-6 months later, serially transplanted three times and then cultured in the presence of cytokines (PubMed=29478914).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Omics: CRISPR phenotypic screen.
Disease Mouse leukemia (NCIt: C21604)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Sex of cell Male
Category Cancer cell line
Publications

PubMed=29478914; DOI=10.1016/j.ccell.2018.01.012; PMCID=PMC5849534
Yamauchi T., Masuda T., Canver M.C., Seiler M., Semba Y., Shboul M., Al-Raqad M., Maeda M., Schoonenberg V.A.C., Cole M.A., Macias-Trevino C., Ishikawa Y., Yao Q.-M., Nakano M., Arai F., Orkin S.H., Reversade B., Buonamici S., Pinello L., Akashi K., Bauer D.E., Maeda T.
Genome-wide CRISPR-Cas9 screen identifies leukemia-specific dependence on a pre-mRNA metabolic pathway regulated by DCPS.
Cancer Cell 33:386-400.e5(2018)

Cross-references
CRISP screens repositories BioGRID_ORCS_Cell_line; 815
Encyclopedic resources Wikidata; Q123030850
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number4