Cellosaurus Tau RD P301L FRET Sensitive Biosensor (CVCL_C8YL)
| Cell line name | Tau RD P301L FRET Sensitive Biosensor |
|---|---|
| Synonyms | HEK293T clone 18 |
| Accession | CVCL_C8YL |
| Resource Identification Initiative | To cite this cell line use: Tau RD P301L FRET Sensitive Biosensor (RRID:CVCL_C8YL) |
| Comments | Characteristics: Transduced with a lentivirus construct EF1a-TauRD(P301L)-RLinker-mTurquoise2-T2A-TauRD(P301L)-RLinker-mNeonGreen-WPRE where TauRD(P301L) is the tau repeat domain (RD) with the disease-associated p.Pro301Leu (p.Pro636Leu) mutation and Rlinker is a (EAAAK)3 rigid linker (PubMed=37553663). Characteristics: Shows a high sensitivity in detecting bioactive tau seeds. These seeds can nucleate the aggregation of the reporter proteins. This aggregation produces a FRET signal (PubMed=37553663). Genetic integration: Method=Transduction; Gene=FPbase; ZRKRV; mNeonGreen (Note=Derivative of Branchiostoma lanceolatum LanYFP). Genetic integration: Method=Transduction; Gene=FPbase; 7AV5G; mTurquoise2 (Note=Tq2, cyan fluorescent protein derived from GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0063 (HEK293T) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=37553663; DOI=10.1186/s13024-023-00643-2; PMCID=PMC10408046 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q123033603 |
| Entry history | |
| Entry creation | 05-Oct-2023 |
| Last entry update | 10-Sep-2024 |
| Version number | 3 |