ID   FS1
AC   CVCL_C8X2
DR   GEO; GSM5209347
DR   GEO; GSM5209348
DR   GEO; GSM5209349
DR   GEO; GSM5209350
DR   GEO; GSM5209351
DR   GEO; GSM5209352
DR   GEO; GSM5209353
DR   GEO; GSM5209354
DR   Wikidata; Q123031314
RX   PubMed=18271004;
RX   PubMed=27688058;
RX   PubMed=34153645;
RX   PubMed=36899312;
CC   Characteristics: Homogenous cell morphology with prominent nucleoli and long cytoplasmic processes. Expresses WT1 and show reversion of the WT1+-KTS isoform ratio. Expresses Sertoli cell markers SOX9, MIC2, AR and INHA. Strongly positive for vimentin, which is expressed in Sertoli cells. Negative for the germ cell marker VASA1 and for the Leydig cell marker LHCGR (Direct_author_submission).
CC   Karyotypic information: 46,XY (PubMed=18271004).
CC   Sequence variation: Mutation; HGNC; 12796; WT1; Simple; c.1447+5G>A (IVS9+5G>A); ClinVar=VCV000003493; Zygosity=Heterozygous (PubMed=18271004).
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Omics: Transcriptome analysis by microarray.
CC   Miscellaneous: Detailed characteristics from personal communication of Schulz, Birte.
CC   Derived from site: In situ; Testis; UBERON=UBERON_0000473.
CC   Cell type: Sertoli cell; CL=CL_0000216.
DI   NCIt; C122805; Frasier syndrome
DI   ORDO; Orphanet_347; Frasier syndrome
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   19Y
CA   Transformed cell line
DT   Created: 05-10-23; Last updated: 10-09-24; Version: 3
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RX   PubMed=18271004; DOI=10.1002/mrd.20889;
RA   Schumacher V., Gueler B., Looijenga L.H.J., Becker J.U., Amann K.,
RA   Engers R., Dotsch J., Stoop H., Schulz W., Royer-Pokora B.;
RT   "Characteristics of testicular dysgenesis syndrome and decreased
RT   expression of SRY and SOX9 in Frasier syndrome.";
RL   Mol. Reprod. Dev. 75:1484-1494(2008).
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RX   PubMed=27688058; DOI=10.1007/s00418-016-1503-y;
RA   Hartmann K., Bennien J., Wapelhorst B., Bakhaus K., Schumacher V.,
RA   Kliesch S., Weidner W., Bergmann M., Geyer J., Fietz D.;
RT   "Current insights into the sulfatase pathway in human testis and
RT   cultured Sertoli cells.";
RL   Histochem. Cell Biol. 146:737-748(2016).
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RX   PubMed=34153645; DOI=10.1016/j.neo.2021.05.008; PMCID=PMC8233172;
RA   Fink C., Baal N., Wilhelm J., Sarode P., Weigel R., Schumacher V.,
RA   Nettersheim D., Schorle H., Schrock C., Bergmann M., Kliesch S.,
RA   Kressin M., Savai R.;
RT   "On the origin of germ cell neoplasia in situ: dedifferentiation of
RT   human adult Sertoli cells in cross talk with seminoma cells in
RT   vitro.";
RL   Neoplasia 23:731-742(2021).
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RX   PubMed=36899312; DOI=10.1186/s12885-023-10696-7; PMCID=PMC10007848;
RA   Schulz B., Schumacher V., Ngezahayo A., Maier-Begandt D., Schadzek N.,
RA   Wilhelm J., Weidner W., Pilatz A., Fietz D., Kliesch S., Schnepel N.,
RA   Hambruch N., Rode K., Langeheine M., Brehm R.;
RT   "Analysis of connexin 43, connexin 45 and N-cadherin in the human
RT   Sertoli cell line FS1 and the human seminoma-like cell line TCam-2 in
RT   comparison with human testicular biopsies.";
RL   BMC Cancer 23:232.1-232.20(2023).
//