Cellosaurus BHK-NS5B-FRLuc (CVCL_C8QX)
| Cell line name | BHK-NS5B-FRLuc |
|---|---|
| Accession | CVCL_C8QX |
| Resource Identification Initiative | To cite this cell line use: BHK-NS5B-FRLuc (RRID:CVCL_C8QX) |
| Comments | Characteristics: Transfected with a (+)FLuc-(-)UTR-RLuc construct that comprises firefly luciferase (FLuc) and Renilla luciferase (RLuc) in reverse orientation flanked by both negative strands of the HCV 5' and 3' UTRs. The FLuc and RLuc reporter proteins are respectively regulated by host polymerase and NS5B polymerase (PubMed=20382106). Characteristics: Transfected with hepatitis C virus NS5B (a RNA-dependent RNA polymerase). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Kidney; UBERON=UBERON_0002113. |
| Species of origin | Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036) |
| Hierarchy | Parent: CVCL_C8QW (BHK-NS5B) |
| Sex of cell | Male |
| Age at sampling | 1D |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=20382106; DOI=10.1016/j.ab.2010.04.004 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q123030789 |
| Entry history | |
| Entry creation | 05-Oct-2023 |
| Last entry update | 10-Sep-2024 |
| Version number | 3 |