ID   HEKzeroT
AC   CVCL_C8CF
SY   clone #8
DR   Wikidata; Q123031597
RX   PubMed=37232541;
WW   https://excellgene.com/services/hek-zero-t/
CC   Group: Serum/protein free medium cell line.
CC   Characteristics: Using CRISPR/Cas9 the SV40 large and small T-antigen coding sequences were deleted thus rendering the cell line CMC compliant (PubMed=37232541).
CC   Characteristics: Grows in suspension growth conditions in animal component-free media.
CC   Doubling time: ~20 hours (PubMed=37232541).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C8CE ! HEKExpress
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 29-06-23; Last updated: 10-09-24; Version: 4
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RX   PubMed=37232541; DOI=10.1002/bit.28414;
RA   Croissant C., Armitano J., Lazuech B., Svec D., Pugin C., Guesdon A.,
RA   Bryan L., Castro A., Neuhaus L., Fonti G., Martinis J., Wurm M.J.,
RA   Wurm F.M., Pino P.;
RT   "A new T-antigen negative HEK293 cell line with improved AAV
RT   productivity.";
RL   Biotechnol. Bioeng. 120:1953-1960(2023).
//