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Cellosaurus MSOD (CVCL_C6VW)

[Text version]
Cell line name MSOD
Synonyms M-SOD; Mesenchymal Sword of Damocles; hTERT-iDS; 196hT-iDS clone 20
Accession CVCL_C6VW
Resource Identification Initiative To cite this cell line use: MSOD (RRID:CVCL_C6VW)
Comments Group: Patented cell line.
Characteristics: Capable to efficiently differentiate toward the osteogenic lineage (PubMed=24561906).
Characteristics: Transduced with a lentiviral vector containing hTERT and eGFP respectively under the control of the CMV and EF1a promoters and a retro-vector containing iCasp9-2A-CD19t under the control of a CMV promoter (PubMed=24561906).
Characteristics: The suicide system of this cell line can be activated by addition of an homodimerizer that activates the apoptosis pathway through the dimerization of iCasp9 (FKBP12 linked by a linker SGGGS to Caspase-9) (PubMed=24561906).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1511; CASP9.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1633; CD19 (Note=With p.Lys324_Arg556del = CD19t).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3711; FKBP1A (Note=With p.Phe37Val).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Cell type: Mesenchymal stem cell of bone marrow; CL=CL_0002540.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C6VV (196hT)
Children:
CVCL_C6VX (MSOD-B)
Sex of cell Female
Age at sampling 51Y
Category Telomerase immortalized cell line
STR profile Source(s): PubMed=24561906

Markers:
AmelogeninX
CSF1PO10,11
D3S135816,17
D5S81811,12
D7S82012
D8S117913,15
D13S31712,14
D16S53912
D18S5112,17
D21S1130,32.2
FGA21,23
Penta D11,13
Penta E10,14
TH019.3
TPOX8
vWA15,16

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Publications

Patent=WO2013186264A1
Bourgine P.-E.
Immortalized mesenchymal stem cells that can be killed through an inducible apoptosis system.
Patent number WO2013186264A1, 19-Dec-2013

PubMed=24561906; DOI=10.1016/j.scr.2013.12.006
Bourgine P.-E., Le Magnen C., Pigeot S., Geurts J., Scherberich A., Martin I.
Combination of immortalization and inducible death strategies to generate a human mesenchymal stromal cell line with controlled survival.
Stem Cell Res. 12:584-598(2014)

PubMed=34486186; DOI=10.1002/adma.202103737
Pigeot S., Klein T., Gullotta F., Dupard S.J., Garcia-Garcia A., Garcia-Garcia A., Prithiviraj S., Lorenzo P., Filippi M., Jaquiery C., Kouba L., Asnaghi M.A., Raina D.B., Dasen B., Isaksson H., Onnerfjord P., Tagil M., Bondanza A., Martin I., Bourgine P.-E.
Manufacturing of human tissues as off-the-shelf grafts programmed to induce regeneration.
Adv. Mater. 33:e2103737.1-e2103737.13(2021)

Cross-references
Encyclopedic resources Wikidata; Q117704716
Entry history
Entry creation21-Mar-2023
Last entry update19-Dec-2024
Version number4