ID   LUMCi004-A-2
AC   CVCL_C6UD
SY   LU99_CLYBL-bxb-v2
DR   BioSamples; SAMEA112164541
DR   hPSCreg; LUMCi004-A-2
DR   Wikidata; Q117704635
RX   PubMed=36495703;
CC   From: Leiden University Medical Center; Leiden; Netherlands.
CC   Population: Caucasian.
CC   Characteristics: Contains a platform to precisely integrate DNA payloads into the cell genome. Using TALEN a STRAIGHT-IN v2 construct (loxP-EBFP2-attP(bxb1)-BleoR-lox257) was introduced in one of the alleles of CLYBL (PubMed=36495703).
CC   Genetic integration: Method=TALEN; Gene=FPbase; DVMQ7; EBFP2 (Note=Blue fluorescent protein derived from GFP).
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Skin; UBERON=UBERON_0002097.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_UK77 ! LUMCi004-A
SX   Female
AG   30-34Y
CA   Induced pluripotent stem cell
DT   Created: 21-03-23; Last updated: 10-09-24; Version: 3
//
RX   PubMed=36495703; DOI=10.1016/j.scr.2022.102991;
RA   Blanch-Asensio A., van der Vaart B., Vinagre M., Groen E.,
RA   Arendzen C.H., Freund C.M.A.H., Geijsen N., Mummery C.L., Davis R.P.;
RT   "Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC
RT   lines for integrating DNA payloads.";
RL   Stem Cell Res. 66:102991-102991(2023).
//